- NEIL Gene Knockouts: Correlation with mutation frequency. ...
NEIL Gene Knockouts: Correlation with mutation frequency. Illustrate your essay with specific examples.
Review of literature
Endogenous and exogenous ROS (reactive oxygen species) are
responsible for causing damage to different cellular components
especially DNA at the rate of 104 molecular lesions per
cell per day. A number of disease etiology mechanisms are tied to
mutations in mitochondrial or nuclear DNA (Bandaru et al, 2002).
The Base Excision Repair mechanism is the first line of defense and
consists of enzymes like NEIL1, NEIL2 and NEIL3 (nei endonuclease
VIII-like 1, 2 and 3 respectively) that facilitate DNA damage
repair (Conlon et al, 2005; Hazra et al, 2002; Morland et al,
2002). Studies have shown that deletion or mutation of genes coding
these enzymes leads to a greater incidence of tumours. These
enzymes function as DNA glycosylase and apyrimidinic/apurinic lyase
catalyse beta and delta-elimination reaction steps in the DNA
around the damaged base, generate 3' terminal phosphate and
interact with DNA ligase III alpha, BER and DNMA Polymerase beta.
Additional function include repair of bubble DNA/single-strand DNA
for oxidised bases, stimulate production of another vital repair
enzyme OGG1 by carrying our beta/delta elimination similar to APE1
at the AP site and excision of excises 5-hydroxyuracil and 3' end
proximal 8-hydroxyguanine lesions.
Sources and data acquisition
Data for the present proposal was collected from PUBMED,
Medline, Biomednet, and other medical databases.