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Quantitative Infrared Spectroscopy used as a tool to monitor the hydrolysis of aspirin

Rapid advances in science and medicine have led to an increase in the demand for chemical and structural information on biological and pharmacological materials. Due to its unique fingerprinting capability, vibrational spectroscopy plays a significant role in providing structural and mechanistic information through the ability that IR spectroscopy offers to identify small changes in the structure of molecules found in a sample. Advances in the field of vibrational spectroscopy across the scientific field, and in particular within pharmacology have lead to advances in the methods used for drug design and development.

Infrared (IR) spectroscopy primarily used in the chemical and physical sciences and in material development, is sensitive to intramolecular bonds, which enables the characterization of complex biological patterns rather than analyzing single monomers. The IR spectra contain information about all molecules within the analyzed medium and the method involves the "examination of the twisting, bending, rotating and vibrational motions of atoms in a molecule." (Linde Gas, web Reference) The method is simple and cost-effective (Backhaus et al, 1998) which enables it as a prospective tool for screening procedures in the pharmaceutical field (Petrich, 2000).

Despite the fact that IR spectroscopy is employed, UV spectroscopy is also used however, the use of flow cells for handling appropriate solvents which are used are often required in order to increase the ability to interpret the information produced in the spectra. The development of FA procedures in the field of IR spectrometry has solved some of the many drawbacks of quantitative determination and have increased the repeatability and accuracy of the determinations the possibility of direct analysis of real samples has been enhanced easy and fast methods for the quantitative quality control of several compounds in the same sample have been provided and the sensitivity of FTIR determination has been increased by means of on-line coupling with concentration techniques. The analysis of reactions occurring during the hydrolysis of the analgesic aspirin can be quantified by the combination of FIA/FTIIR.

Hence, it has been shown how it is possible to obtain information regarding specific molecular and mechanistic interactions and structure through the use of vibrational Infrared imaging technique, which will have major implications in pharmaceutical research and practice.

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