- Role of EBV infected lymphocytes in the pathogenesis of Na...
Role of EBV infected lymphocytes in the pathogenesis of Nasopharyngeal carcinoma
We investigated the association between EBV-infected lymphocytes
and the pathogenesis of Nasopharyngeal carcinoma (NPC). In situ
hybridisation (ISH) and Immunohisto chemistry (IHC) were used to
test the frequency and distribution of EBV-infected lymphocytes in
specimens of tonsils from patients with NPC. In situ hybridisation
was used for EBV encoded RNAs (EBER) and Immunohistochemical
expression of LMP-1 protein was examined by Immunohistochemistry in
formalin- fixed, paraffin embedded specimens of NPC.
I would like to highlight some of the errors in our study. I
think that the EBERs-positive epithelial cells could have been
confirmed better by double staining of cytokeratine and also it is
important to remember that the comparing the frequencies of the
virus in few slides of the NPC case's tonsils is very different
from comparing the frequency in several people. The latter is more
representative and relevant in terms of sampling.
Optimum use of IHC and ISH is very important in studies like
these and when used effectively these methods can yield great
results. I would like to further emphasise on the effective use and
intended benefits of IHC and ISH by following examples.