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Role of EBV infected lymphocytes in the pathogenesis of Nasopharyngeal carcinoma

Summary

We investigated the association between EBV-infected lymphocytes and the pathogenesis of Nasopharyngeal carcinoma (NPC). In situ hybridisation (ISH) and Immunohisto chemistry (IHC) were used to test the frequency and distribution of EBV-infected lymphocytes in specimens of tonsils from patients with NPC. In situ hybridisation was used for EBV encoded RNAs (EBER) and Immunohistochemical expression of LMP-1 protein was examined by Immunohistochemistry in formalin- fixed, paraffin embedded specimens of NPC.

I would like to highlight some of the errors in our study. I think that the EBERs-positive epithelial cells could have been confirmed better by double staining of cytokeratine and also it is important to remember that the comparing the frequencies of the virus in few slides of the NPC case's tonsils is very different from comparing the frequency in several people. The latter is more representative and relevant in terms of sampling.

Optimum use of IHC and ISH is very important in studies like these and when used effectively these methods can yield great results. I would like to further emphasise on the effective use and intended benefits of IHC and ISH by following examples.

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