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Method for measurement of glucose concentrations


It is essential that everyone keeps track of the amount of glucose that circulates around the body. In particular, people who suffer from Diabetes Mellitus or have a history of Diabetes in their family have to be more concerned with the amount of glucose in their body. There are two main bodily fluids, namely urine and blood that may be used to measure the glucose level in the body. However, measurements of glucose in the blood have been found to be more accurate than urine glucose tests. Monitoring of blood glucose is especially fundamental for diabetics who need to know whether their diabetes is under control. It is expressed as millimoles per litre (mmol/l) and the normal blood glucose level usually stays within the narrow limits throughout the day of 4 to 8mmol/l (Henry J.B. 2001). The blood glucose levels are expected be the highest after meals. Blood glucose levels which are above the normal levels for long periods of time usually are at high risk of developing serious complications that are associated with diabetes.


As mentioned above, this method is not 100% accurate and as such gives a rough gauge of the concentration of glucose in the blood sample. A more accurate measure of the glucose in the blood can be obtained using an electronic blood glucose meter. However, if the method of the present experiment is preferred, the results obtained may be made more accurate by increasing the number of references so that the measure of glucose concentration in the blood is more accurate to the decimal. Further, instead of lysing the blood cells, it may be more appropriate to centrifuge the whole blood and remove the blood cells, thus only measuring the blood glucose level in the serum. This may overcome the disadvantages attributed by the presence of the blood cells.

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